The story picks up with Pico and his friend Chico in a sprawling city. Their lives take a turn when they encounter a beautiful, long-haired boy named CoCo, who looks so much like a girl that Pico is initially fooled.

Reaction videos spawned widespread catchphrases, including "Don't watch an anime called Boku" and the emphatic exclamation of "THREEEEEE!" —which explicitly referenced the climax of the Pico x Coco x Chico episode.

The cultural significance of "Pico Coco Chico Ova" lies in its representation of traditional values and culinary practices. It embodies the essence of a culture that prioritizes health, sustainability, and community. As it travels across borders, it not only brings a taste of its homeland to international consumers but also fosters cultural exchange and understanding.

CoCo introduces them to his unconventional home—a series of hidden rooms in an abandoned subway station, setting a unique and atmospheric stage. Here, Pico has a late-night realization about CoCo's true nature, discovering "his" real gender. What follows is a psychological and erotic exploration of attraction and jealousy.

: A slightly younger, energetic boy whom Pico met and initiated into sexual activity during the second OVA, Pico to Chico .